![](/images/graphics-bg.png)
MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7
Joint Authors
Liu, Shikun
Song, Li-Ying
Ma, Yu-Tao
Wu, Cui-Fang
Wang, Chun-Jiang
Fang, Wei-Jin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background and Aim.
Aberrant activation of the TGF-β1/Smad pathway contributes to the activation of hepatic stellate cells (HSCs).
MicroRNA-195 has been shown to regulate the activation of HSCs.
The aim of this study was to investigate the role of miRNA-195 in HSCs activation.
Methods.
A liver fibrotic rat model induced by diethylnitrosamine was established.
Dual luciferase reporter assays were performed to verify that Smad7 was the target of miRNA-195.
The expression levels of miR-195, Smad7, and α-SMA in HSC-T6 transfected, respectively, with miR-195 mimic, inhibitor, or control were measured by qRT-PCR.
The protein expression of Smad7 was detected by Western blot analysis.
Results.
Enhanced miR-195 and decreased Smad7 were observed in diethylnitrosamine-induced liver fibrotic rats (P<0.05).
Dual luciferase reporter assays showed that the miR-195 mimic significantly suppressed the luciferase activity of a reporter plasmid carrying the binding site of miR-195 on the 3′UTR of Smad7 (P<0.05).
The miR-195 mimics activated HSCs, further elevated miR-195 and α-SMA (P<0.01), and reduced the Smad7 level (P<0.05).
The miR-195 inhibitors blocked the activation of HSCs, reduced the expression of miR-195 and α-SMA (P<0.01), and upregulated the expression of Smad7 (P<0.05).
Conclusion.
Collectively, we demonstrated that miRNA-195 activated HSCs by targeting Smad7.
American Psychological Association (APA)
Song, Li-Ying& Ma, Yu-Tao& Wu, Cui-Fang& Wang, Chun-Jiang& Fang, Wei-Jin& Liu, Shikun. 2017. MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134348
Modern Language Association (MLA)
Song, Li-Ying…[et al.]. MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1134348
American Medical Association (AMA)
Song, Li-Ying& Ma, Yu-Tao& Wu, Cui-Fang& Wang, Chun-Jiang& Fang, Wei-Jin& Liu, Shikun. MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134348
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134348