Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals
Joint Authors
Gelinsky, Michael
Heiss, Christian
Thormann, Ulrich
Alt, Volker
Kern, Stefanie
El Khassawna, Thaqif
Malhan, Deeksha
Hassan, Fathi
Daghma, Diaa Eldin S.
Stoetzel, Sabine
Ray, Seemun
Henß, Anja
Rohnke, Marcus
Stengel, Annette
Maenz, Stefan
Jandt, Klaus D.
Diefenbeck, Michael
Schumacher, Matthias
Lips, Katrin Susanne
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Bone histology of decalcified or undecalcified samples depends on the investigation.
However, in research each method provides different information to answer the scientific question.
Decalcification is the first step after sample fixation and governs what analysis is later feasible on the sections.
Besides, decalcification is favored for immunostaining and in situ hybridization.
Otherwise, sample decalcification can be damaging to bone biomaterials implants that contains calcium or strontium.
On the other hand, after decalcification mineralization cannot be assessed using histology or imaging mass spectrometry.
The current study provides a solution to the hardship caused by material presence within the bone tissue.
The protocol presents a possibility of gaining sequential and alternating decalcified and undecalcified sections from the same bone sample.
In this manner, investigations using histology, protein signaling, in situ hybridization, and mass spectrometry on the same sample can better answer the intended research question.
Indeed, decalcification of sections and grindings resulted in well-preserved sample and biomaterials integrity.
Immunostaining was comparable to that of classically decalcified samples.
The study offers a novel approach that incites correlative analysis on the same sample and reduces the number of processed samples whether clinical biopsies or experimental animals.
American Psychological Association (APA)
El Khassawna, Thaqif& Daghma, Diaa Eldin S.& Stoetzel, Sabine& Ray, Seemun& Kern, Stefanie& Malhan, Deeksha…[et al.]. 2017. Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1134427
Modern Language Association (MLA)
El Khassawna, Thaqif…[et al.]. Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1134427
American Medical Association (AMA)
El Khassawna, Thaqif& Daghma, Diaa Eldin S.& Stoetzel, Sabine& Ray, Seemun& Kern, Stefanie& Malhan, Deeksha…[et al.]. Postembedding Decalcification of Mineralized Tissue Sections Preserves the Integrity of Implanted Biomaterials and Minimizes Number of Experimental Animals. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1134427
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134427