Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells
Joint Authors
Zhang, Lian-Dong
Li, He-Cheng
Gao, Ming
Zhang, Tongdian
Chong, Tie
Wang, Ziming
Zhai, Xiaoqiang
Wu, Zhizhong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mono-(2-ethylhexyl) phthalate (MEHP) and genistein are two of the most prevalent endocrine-disrupting chemicals (EDCs) that present in the environment and food.
However, how these two EDCs would affect prepubertal Sertoli cells development was rarely studied.
In this study, primary prepubertal Sertoli cells were isolated from 22-day-old Sprague Dawley rats and exposed to MEHP at 1 μmol/L, 10 μmol/L, and 100 μmol/L (M1, M10, and M100), genistein at 10 μmol/L (G), and their combination (G + M1, G + M10, and G + M100).
Cell proliferation inhibition rate, apoptosis and necrosis rate, and cellular redox state were evaluated.
Our results revealed that MEHP could significantly increase cell proliferation inhibition rate, apoptosis rate, necrosis rate, and intracellular reactive oxidative species level.
However, coadministration of genistein could partially alleviate MEHP-induced prepubertal Sertoli cells oxidative injuries via enhancement of testicular antioxidative enzymes activities and upregulation of Nrf2 and HO-1, indicating that genistein could partially attenuate MEHP-induced prepubertal Sertoli cells damage through antioxidative action and may have promising future on its curative role for attenuating other EDCs-induced reproductive disorders.
American Psychological Association (APA)
Zhang, Lian-Dong& Gao, Ming& Zhang, Tongdian& Chong, Tie& Wang, Ziming& Zhai, Xiaoqiang…[et al.]. 2017. Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134446
Modern Language Association (MLA)
Zhang, Lian-Dong…[et al.]. Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1134446
American Medical Association (AMA)
Zhang, Lian-Dong& Gao, Ming& Zhang, Tongdian& Chong, Tie& Wang, Ziming& Zhai, Xiaoqiang…[et al.]. Protective Effects of Genistein against Mono-(2-ethylhexyl) Phthalate-Induced Oxidative Damage in Prepubertal Sertoli Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134446