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Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines
Joint Authors
Wang, Qian
Xu, Pengju
Ren, Luyao
Du, Yonggang
Zong, Wansong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
As potential inhibitors target to biological enzymes, antibiotics may have certain impacts on the biochemical treatment process.
With micrococcus catalase (CAT) served as the target molecule, the impact and inhibition mechanism for typical tetracyclines (TCs) were evaluated.
Toxicity experiments showed that TCs had significant inhibition on CAT in the sequence of tetracycline>chlortetracycline>oxytetracycline>doxycycline.
To clarify the inhibition mechanism between TCs and CAT which was explored with the assistance of fluorescence spectroscopy and MOE molecule simulation.
According to fluorescence analysis, TCs quenched the fluorescence signal of CAT by the mode of static quenching.
Combined with toxicity data, it could be presumed that TCs combined with the catalytic active center and thus inhibited CAT.
Above presumption was further verified by the molecular simulation data.
When TCs combined with the catalytic center of CAT, the compounds have increased combination areas and prominent energy change (compared with the compounds formed by TCs and noncatalytic center recommend by MOE software).
IBM SPSS statistics showed that TC toxicity positively correlated with the hydrogen bonds such as O13→Glu252, O1←Arg195, and O6→Asp249, but negatively correlated with the hydrogen bonds such as O10→Pro363, O10→Lys455, and O12 → Asn127.
TC toxicity also positively correlated with the ion bonds ofN4-Glu252, but negatively correlated with the ion bonds of N4-Asp379.
Hydrogen bonds and ion bonds for above key sites were closely related to the inhibition effect of TCs on CAT.
American Psychological Association (APA)
Ren, Luyao& Wang, Qian& Du, Yonggang& Xu, Pengju& Zong, Wansong. 2020. Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1134450
Modern Language Association (MLA)
Ren, Luyao…[et al.]. Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1134450
American Medical Association (AMA)
Ren, Luyao& Wang, Qian& Du, Yonggang& Xu, Pengju& Zong, Wansong. Research on the Impact and Mechanism for the Inhibition of Micrococcus Catalase Activity by Typical Tetracyclines. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1134450
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134450