Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3KAktmTOR Signaling Pathway
Joint Authors
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To investigate whether Polyphyllin I (PPI) might induce the autophagy and apoptosis of melanoma cells by regulating PI3K/Akt/mTOR signal pathway.
Melanoma A375 cells were incubated with different concentrations of Polyphyllin I (0, 1.5, 3.0, and 6.0 mg/L) and PI3K/Akt/mTOR signaling pathway activator IGF-1(20 mg/L).
CCK-8 assay was utilized to detect cell proliferation; Cell apoptosis and cell cycle were measured by flow cytometry; Western blot was used to examine the expressions of proteins.
Immunofluorescence analysis was performed to evaluate autophagy of A375 cells; In addition, xenograft-bearing nude mice were applied to study the role of Polyphyllin I on melanoma development, melanoma cell proliferation, as well as melanoma cell apoptosis in vivo.
The outcomes represented that Polyphyllin I promoted A375 cell apoptosis via upregulating Bax level and cleaved caspase-3 level and downregulating Bcl-2 level, inhibited the growth of A375 cells at the G0/G1 phase, and enhanced cell autophagy via regulating the levels of Beclin 1, LC3II, and p62.
However, IGF-1 (an activator of PI3K/Akt/mTOR signal pathway) attenuated these changes that Polyphyllin I induced.
Furthermore, the xenograft model experiment confirmed that Polyphyllin I treatment suppressed xenograft tumor growth, increased apoptotic index evaluated by the TUNEL method, and reduced the level of Ki67 in tumor tissues in vivo.
In conclusion, Polyphyllin I treatment enhanced melanoma cell autophagy and apoptosis, as well as blocked melanoma cell cycle via suppressing PI3K/Akt/mTOR signal pathway.
Meanwhile, Polyphyllin I treatment suppressed the development of melanoma in vivo.
Therefore, Polyphyllin I possibly is a promising molecular targeted agent used in melanoma therapy.
American Psychological Association (APA)
Long, Jianwen& Pi, Xianming. 2020. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3KAktmTOR Signaling Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134516
Modern Language Association (MLA)
Long, Jianwen& Pi, Xianming. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3KAktmTOR Signaling Pathway. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134516
American Medical Association (AMA)
Long, Jianwen& Pi, Xianming. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3KAktmTOR Signaling Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134516