Comprehensive Analyses of miRNA-mRNA Network and Potential Drugs in Idiopathic Pulmonary Arterial Hypertension
Joint Authors
Li, Chan
Zhang, Zeyu
Xu, Qian
Shi, Ruizheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Idiopathic pulmonary arterial hypertension (IPAH) is a severe cardiopulmonary disease with a relatively low survival rate.
Moreover, the pathogenesis of IPAH has not been fully recognized.
Thus, comprehensive analyses of miRNA-mRNA network and potential drugs in IPAH are urgent requirements.
Methods.
Microarray datasets of mRNA and microRNA (miRNA) in IPAH were searched and downloaded from Gene Expression Omnibus (GEO).
Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMIs) were identified.
Then, the DEMI-DEG network was conducted with associated comprehensive analyses including Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis, while potential drugs targeting hub genes were investigated using L1000 platform.
Results.
30 DEGs and 6 DEMIs were identified in the lung tissue of IPAH.
GO and KEGG pathway analyses revealed that these DEGs were mostly enriched in antimicrobial humoral response and African trypanosomiasis, respectively.
The DEMI-DEG network was conducted subsequently with 4 DEMIs (hsa-miR-34b-5p, hsa-miR-26b-5p, hsa-miR-205-5p, and hsa-miR-199a-3p) and 16 DEGs, among which 5 DEGs (AQP9, SPP1, END1, VCAM1, and SAA1) were included in the top 10 hub genes of the PPI network.
Nimodipine was identified with the highest CMap connectivity score in L1000 platform.
Conclusion.
Our study conducted a miRNA-mRNA network and identified 4 miRNAs as well as 5 mRNAs which may play important roles in the pathogenesis of IPAH.
Moreover, we provided a new insight for future therapies by predicting potential drugs targeting hub genes.
American Psychological Association (APA)
Li, Chan& Zhang, Zeyu& Xu, Qian& Shi, Ruizheng. 2020. Comprehensive Analyses of miRNA-mRNA Network and Potential Drugs in Idiopathic Pulmonary Arterial Hypertension. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134522
Modern Language Association (MLA)
Li, Chan…[et al.]. Comprehensive Analyses of miRNA-mRNA Network and Potential Drugs in Idiopathic Pulmonary Arterial Hypertension. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1134522
American Medical Association (AMA)
Li, Chan& Zhang, Zeyu& Xu, Qian& Shi, Ruizheng. Comprehensive Analyses of miRNA-mRNA Network and Potential Drugs in Idiopathic Pulmonary Arterial Hypertension. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134522
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134522