Identification and Validation of Prognostically Relevant Gene Signature in Melanoma
Joint Authors
Chen, Hong-Duo
Song, Bing
Gao, Yali
Li, Yaling
Niu, Xueli
Wu, Yutong
Guan, Xiuhao
Hong, Yuxiao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-29, 29 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-11
Country of Publication
Egypt
No. of Pages
29
Main Subjects
Abstract EN
Background.
Currently, effective genetic markers are limited to predict the clinical outcome of melanoma.
High-throughput multiomics sequencing data have provided a valuable approach for the identification of genes associated with cancer prognosis.
Method.
The multidimensional data of melanoma patients, including clinical, genomic, and transcriptomic data, were obtained from The Cancer Genome Atlas (TCGA).
These samples were then randomly divided into two groups, one for training dataset and the other for validation dataset.
In order to select reliable biomarkers, we screened prognosis-related genes, copy number variation genes, and SNP variation genes and integrated these genes to further select features using random forests in the training dataset.
We screened for robust biomarkers and established a gene-related prognostic model.
Finally, we verified the selected biomarkers in the test sets (GSE19234 and GSE65904) and on clinical samples extracted from melanoma patients using qRT-PCR and immunohistochemistry analysis.
Results.
We obtained 1569 prognostic-related genes and 1101 copy-amplification, 1093 copy-deletions, and 92 significant mutations in genomic variants.
These genomic variant genes were closely related to the development of tumors and genes that integrate genomic variation.
A total of 141 candidate genes were obtained from prognosis-related genes.
Six characteristic genes (IQCE, RFX6, GPAA1, BAHCC1, CLEC2B, and AGAP2) were selected by random forest feature selection, many of which have been reported to be associated with tumor progression.
Cox regression analysis was used to establish a 6-gene signature.
Experimental verification with qRT-PCR and immunohistochemical staining proved that these selected genes were indeed expressed at a significantly higher level compared with the normal tissues.
This signature comprised an independent prognostic factor for melanoma patients.
Conclusions.
We constructed a 6-gene signature (IQCE, RFX6, GPAA1, BAHCC1, CLEC2B, and AGAP2) as a novel prognostic marker for predicting the survival of melanoma patients.
American Psychological Association (APA)
Gao, Yali& Li, Yaling& Niu, Xueli& Wu, Yutong& Guan, Xiuhao& Hong, Yuxiao…[et al.]. 2020. Identification and Validation of Prognostically Relevant Gene Signature in Melanoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-29.
https://search.emarefa.net/detail/BIM-1134692
Modern Language Association (MLA)
Gao, Yali…[et al.]. Identification and Validation of Prognostically Relevant Gene Signature in Melanoma. BioMed Research International No. 2020 (2020), pp.1-29.
https://search.emarefa.net/detail/BIM-1134692
American Medical Association (AMA)
Gao, Yali& Li, Yaling& Niu, Xueli& Wu, Yutong& Guan, Xiuhao& Hong, Yuxiao…[et al.]. Identification and Validation of Prognostically Relevant Gene Signature in Melanoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-29.
https://search.emarefa.net/detail/BIM-1134692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134692