Dipeptidyl Peptidase-4 Is a Target Protein of Epigallocatechin-3-Gallate
Joint Authors
Wang, Jian
Hou, Huimin
Wang, Ying
Li, Chunshi
Cao, Yanli
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Epigallocatechin-3-gallate (EGCG), a major active ingredient in green tea, has various health benefits.
It affects glucose metabolism, but the mechanism is not well understood.
This study aimed to identify targets of EGCG related to glucose metabolism.
The core fragment of EGCG is a flavonoid.
The flavonoid scaffold was used as a substructure to find proteins cocrystallized with flavonoids in the Protein Data Bank.
The proteins identified were screened in PubMed for known relationships with diabetes.
Dipeptidyl peptidase-4 (DPP4; PDB 5J3J) was identified following this approach.
By molecular docking, the interactions of EGCG and DPP4 were assessed.
To test the stability of the interactions between EGCG and DPP4, molecular dynamics simulation for 100 ns was performed using Desmond software.
In vitro, the concentration of EGCG required to inhibit DPP4 activity by 50% (the IC50 value) was 28.42 μM.
These data provide a theoretical basis for intervention in glucose metabolism with EGCG.
American Psychological Association (APA)
Hou, Huimin& Wang, Ying& Li, Chunshi& Wang, Jian& Cao, Yanli. 2020. Dipeptidyl Peptidase-4 Is a Target Protein of Epigallocatechin-3-Gallate. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134726
Modern Language Association (MLA)
Hou, Huimin…[et al.]. Dipeptidyl Peptidase-4 Is a Target Protein of Epigallocatechin-3-Gallate. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134726
American Medical Association (AMA)
Hou, Huimin& Wang, Ying& Li, Chunshi& Wang, Jian& Cao, Yanli. Dipeptidyl Peptidase-4 Is a Target Protein of Epigallocatechin-3-Gallate. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134726