Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data
Joint Authors
Xiong, Yongfu
You, Wenxian
Wang, Rong
Peng, Linglong
Fu, Zhongxue
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Although hundreds of colorectal cancer- (CRC-) related genes have been screened, the significant hub genes still need to be further identified.
The aim of this study was to identify the hub genes based on protein-protein interaction network and uncover their clinical value.
Firstly, 645 CRC patients’ data from the Tumor Cancer Genome Atlas were downloaded and analyzed to screen the differential expression genes (DEGs).
And then, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed, and PPI network of the DEGs was constructed by Cytoscape software.
Finally, four hub genes (CXCL3, ELF5, TIMP1, and PHLPP2) were obtained from four subnets and further validated in our clinical setting and TCGA dataset.
The results showed that mRNA expression of CXCL3, ELF5, and TIMP1 was increased in CRC tissues, whereas PHLPP2 mRNA expression was decreased.
More importantly, high expression of CXCL3, ELF5, and TIMP1 was significantly associated with lymphatic invasion, distance metastasis, and advanced tumor stage.
In addition, a shorter overall survival was observed in patients with increased CXCL3, TIMP1, and ELF5 expression and decreased PHLPP2 expression.
In conclusion, the four hub genes screened by our strategy could serve as novel biomarkers for prognosis prediction of CRC patients.
American Psychological Association (APA)
Xiong, Yongfu& You, Wenxian& Wang, Rong& Peng, Linglong& Fu, Zhongxue. 2017. Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134727
Modern Language Association (MLA)
Xiong, Yongfu…[et al.]. Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1134727
American Medical Association (AMA)
Xiong, Yongfu& You, Wenxian& Wang, Rong& Peng, Linglong& Fu, Zhongxue. Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1134727
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134727