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Amarogentin Inhibits Liver Cancer Cell Angiogenesis after Insufficient Radiofrequency Ablation via Affecting Stemness and the p53-Dependent VEGFADll4Notch1 Pathway
Joint Authors
Zhang, Yongchuan
Zhang, Yinglin
Wang, Jin
Gu, Haitao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Whether and how amarogentin suppresses the angiogenesis effect in liver cancer cells after insufficient radiofrequency ablation (iRFA) are still poorly studied.
Methods.
The number of liver cancer stem cells (LCSCs) and the level of vascular endothelial growth factor A (VEGFA) were assessed in liver cancer tissue after iRFA.
Then, CD133-positive cells were detected in iRFA models of HepG2 and Huh7 cell lines treated with amarogentin.
Tube formation assays were applied to observe the antiangiogenesis effects of amarogentin.
In addition, the angiogenesis-related molecules p53, delta-like ligand 4 (Dll4), and Notch1 were detected in the iRFA cells and mouse models treated with amarogentin.
Results.
The mRNA and protein expression levels of CD133 and VEGFA were significantly higher in the residual liver cancer tissue than in the liver cancer tissues treated by hepatectomy.
Amarogentin then markedly decreased the percentage of CD133-positive cells in the iRFA model in both HepG2 and Huh7 cell lines.
The number of tubules formed by human umbilical vein endothelial cells (HUVECs) was significantly decreased by amarogentin.
Inversely, the antiangiogenesis effect of amarogentin was counteracted after p53 silencing in the iRFA cell models.
Conclusion.
Amarogentin prevents the malignant transformation of liver cancer after iRFA via affecting stemness and the p53-dependent VEGFA/Dll4/Notch1 pathway to inhibit cancer cell angiogenesis.
American Psychological Association (APA)
Zhang, Yongchuan& Zhang, Yinglin& Wang, Jin& Gu, Haitao. 2020. Amarogentin Inhibits Liver Cancer Cell Angiogenesis after Insufficient Radiofrequency Ablation via Affecting Stemness and the p53-Dependent VEGFADll4Notch1 Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134737
Modern Language Association (MLA)
Zhang, Yongchuan…[et al.]. Amarogentin Inhibits Liver Cancer Cell Angiogenesis after Insufficient Radiofrequency Ablation via Affecting Stemness and the p53-Dependent VEGFADll4Notch1 Pathway. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1134737
American Medical Association (AMA)
Zhang, Yongchuan& Zhang, Yinglin& Wang, Jin& Gu, Haitao. Amarogentin Inhibits Liver Cancer Cell Angiogenesis after Insufficient Radiofrequency Ablation via Affecting Stemness and the p53-Dependent VEGFADll4Notch1 Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1134737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134737