Chromodomain Helicase DNA-Binding Protein 5 Inhibits Renal Cell Carcinoma Tumorigenesis by Activation of the p53 and RB Pathways
Joint Authors
Huang, Sheng
Yan, Qitao
Xiong, Shilin
Peng, Yiqi
Zhao, Rui
Liu, Chunxiao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Chromodomain helicase DNA-binding protein 5 (CHD5) plays a crucial tumor suppressor role in multiple types of tumors.
For this study, we investigated its clinical significance and the molecular mechanism(s) underlying tumorigenesis in renal cell carcinoma (RCC).
Initially, CHD5 expression was assessed in primary tumor tissue and in tissue array.
Correlations among CHD5 expression and clinicopathological characteristics were analyzed.
Next, lentivirus-mediated CHD5 overexpression in the ACHN and 769-P cells was used to assess effects on proliferation, migration, invasion ability, and the regulation of the p14ARF/p53 and p16INK4a/RB signaling pathways.
Finally, a xenograft mouse model was used to verify its impact on tumor growth in vivo.
Results demonstrated that CHD5 was downregulated in tumor tissues and that low CHD5 expression was correlated with advanced TNM stage, high Fuhrman grade, lymph node metastasis, and poor survival.
Overexpression of CHD5 inhibited proliferation, migration, and invasion in vitro; prompted cell cycle G1 phase arrest; induced apoptosis; and suppressed tumor growth in vivo.
Furthermore, we confirmed that CHD5 activates the p53 and RB pathways to inhibit tumorigenesis in RCC.
In summary, CHD5 is involved in the initiation and progression of RCC and may serve as a diagnostic biomarker and a potential therapeutic target for RCC.
American Psychological Association (APA)
Huang, Sheng& Yan, Qitao& Xiong, Shilin& Peng, Yiqi& Zhao, Rui& Liu, Chunxiao. 2020. Chromodomain Helicase DNA-Binding Protein 5 Inhibits Renal Cell Carcinoma Tumorigenesis by Activation of the p53 and RB Pathways. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1134763
Modern Language Association (MLA)
Huang, Sheng…[et al.]. Chromodomain Helicase DNA-Binding Protein 5 Inhibits Renal Cell Carcinoma Tumorigenesis by Activation of the p53 and RB Pathways. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1134763
American Medical Association (AMA)
Huang, Sheng& Yan, Qitao& Xiong, Shilin& Peng, Yiqi& Zhao, Rui& Liu, Chunxiao. Chromodomain Helicase DNA-Binding Protein 5 Inhibits Renal Cell Carcinoma Tumorigenesis by Activation of the p53 and RB Pathways. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1134763
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134763