Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45

Joint Authors

Kovács, Tamás
Pertics, Botond Zsombor
Szénásy, Dalma
Dunai, Dániel
Born, Yannick
Fieseler, Lars
Schneider, György

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-23

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Methicillin-resistant Staphylococcus aureus (MRSA) can cause a wide range of infections from mild to life-threatening conditions.

Its enhanced antibiotic resistance often leads to therapeutic failures and therefore alternative eradication methods must be considered.

Potential candidates to control MRSA infections are bacteriophages and their lytic enzymes, lysins.

In this study, we isolated a bacteriophage against a nosocomial MRSA strain belonging to the ST45 epidemiologic group.

The phage belonging to Caudovirales, Siphoviridae, showed a narrow host range and stable lytic activity without the emergence of resistant MRSA clones.

Phylogenetic analysis showed that the newly isolated Staphylococcus phage R4 belongs to the Triavirus genus in Siphoviridae family.

Genetic analysis of the 45 kb sequence of R4 revealed 69 ORFs.

No remnants of mobile genetic elements and traces of truncated genes were observed.

We have localized the lysin (N-acetylmuramoyl-L-alanine amidase) gene of the new phage that was amplified, cloned, expressed, and purified.

Its activity was verified by zymogram analysis.

Our findings could potentially be used to develop specific anti-MRSA bacteriophage- and phage lysin-based therapeutic strategies against major clonal lineages and serotypes.

American Psychological Association (APA)

Pertics, Botond Zsombor& Szénásy, Dalma& Dunai, Dániel& Born, Yannick& Fieseler, Lars& Kovács, Tamás…[et al.]. 2020. Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134794

Modern Language Association (MLA)

Pertics, Botond Zsombor…[et al.]. Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1134794

American Medical Association (AMA)

Pertics, Botond Zsombor& Szénásy, Dalma& Dunai, Dániel& Born, Yannick& Fieseler, Lars& Kovács, Tamás…[et al.]. Isolation of a Novel Lytic Bacteriophage against a Nosocomial Methicillin-Resistant Staphylococcus aureus Belonging to ST45. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1134794

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1134794