Mutagenic and Cytotoxicity LQB 123 Profile: Safety and Tripanocidal Effect of a Phenyl-t-Butylnitrone Derivative
Joint Authors
Menna-Barreto, Rubem Figueiredo Sadok
Nogueira, Natália Pereira de Almeida
Felzenszwalb, Israel
Cupello, Mauricio Peixoto
Saraiva, Francis Monique
Ippolito, Pedro
Fernandes, Andréia da Silva
Costa, Debora de Sousa dos Santos
Paula, Jessica Isis Oliveira
Costa, Paulo Roberto Ribeiro
Dias, Ayres Guimarães
Paes, Marcia Cristina
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The therapeutic options for Chagas disease are limited and its treatment presents a number of drawbacks including toxicity, drug resistance, and insufficient effectiveness against the chronic stage of the disease.
Therefore, new therapeutical options are mandatory.
In the present work, we evaluated the effect of a phenyl-tert-butylnitrone (PBN) derivate, LQB 123, against Trypanosoma cruzi forms.
LQB 123 presented a trypanocidal effect against bloodstream trypomastigotes (IC50 = 259.4±6.1 μM) and intracellular amastigotes infecting peritoneal macrophages (IC50 = 188.2±47.5 μM), with no harmful effects upon the mammalian cells (CC50 values greater than 4 mM), resulting in a high selectivity index (CC50/IC50 > 20).
Additionally, metacyclic trypomastigotes submitted to LQB 123 presented an IC50 of about 191.8±10.5 μM and epimastigotes forms incubated with different concentrations of LQB 123 presented an inhibition of parasite growth with an IC50 of 255.1±3.6 μM.
Finally, we investigated the mutagenic potential of the nitrone by the Salmonella/microsome assay and observed no induction of mutagenicity even in concentrations as high as 33000 μM.
Taken together, these results present a nonmutagenic compound, with trypanocidal activity against all relevant forms of T.
cruzi, offering new insights into CD treatment suggesting additional in vivo tests.
American Psychological Association (APA)
Cupello, Mauricio Peixoto& Saraiva, Francis Monique& Ippolito, Pedro& Fernandes, Andréia da Silva& Menna-Barreto, Rubem Figueiredo Sadok& Costa, Debora de Sousa dos Santos…[et al.]. 2017. Mutagenic and Cytotoxicity LQB 123 Profile: Safety and Tripanocidal Effect of a Phenyl-t-Butylnitrone Derivative. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1134811
Modern Language Association (MLA)
Cupello, Mauricio Peixoto…[et al.]. Mutagenic and Cytotoxicity LQB 123 Profile: Safety and Tripanocidal Effect of a Phenyl-t-Butylnitrone Derivative. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1134811
American Medical Association (AMA)
Cupello, Mauricio Peixoto& Saraiva, Francis Monique& Ippolito, Pedro& Fernandes, Andréia da Silva& Menna-Barreto, Rubem Figueiredo Sadok& Costa, Debora de Sousa dos Santos…[et al.]. Mutagenic and Cytotoxicity LQB 123 Profile: Safety and Tripanocidal Effect of a Phenyl-t-Butylnitrone Derivative. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1134811
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134811