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Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen
Joint Authors
Wang, Long-Bai
Chen, Qiu-Yong
Wu, Xue-Min
Che, Yong-Liang
Wang, Cheng-Yan
Chen, Ru-Jing
Zhou, Lun-Jiang
Yan, Shan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study described a TaqMan based real-time fluorescent quantitative PCR (qPCR) method to detect porcine cytomegalovirus (PCMV) infection, targeting the conserved region of the DNA polymerase (DPOL) gene.
The standard curve showed a linear regression relationship with a coefficient of 0.999 and a slope of y = −3.249x + 38.958 corresponding to the amplification efficiency at 99.8%.
The limit of the qPCR method was 51.9 copies/μl.
The established qPCR method showed excellent specificity, with no cross-reaction observed with common porcine pathogens.
The coefficient of variation for intra-assay and interassay variability ranged up to 1.51% and 2.24%, respectively.
PCMV positive signals can be found in semen using this qPCR method, which suggested that we should pay more attention to PCMV contamination in semen in order to eliminate PCMV infection in artificial insemination and xenotransplantation.
American Psychological Association (APA)
Chen, Ru-Jing& Chen, Qiu-Yong& Wu, Xue-Min& Che, Yong-Liang& Wang, Cheng-Yan& Wang, Long-Bai…[et al.]. 2020. Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1134870
Modern Language Association (MLA)
Chen, Ru-Jing…[et al.]. Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1134870
American Medical Association (AMA)
Chen, Ru-Jing& Chen, Qiu-Yong& Wu, Xue-Min& Che, Yong-Liang& Wang, Cheng-Yan& Wang, Long-Bai…[et al.]. Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1134870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134870