Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats
Joint Authors
Yan-Ping, Dai
Xiao-Qin, Gao
Xiao-Ping, Ma
Ying-Quan, Yue
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
To study the expressions of VEGF and VEGFR2 at protein level in the epididymis of rats with arsenism.
Methods.
Forty male Sprague-Dawley rats were randomly divided into four groups: the high dose arsenic infected group (60.0 mg/L in water), the middle dose arsenic infected group (12.0 mg/L in water), the low dose arsenic infected group (2.4 mg/L in water), and the control group (distilled water).
Rats were treated with arsenic through drinking water for 6 consecutive months.
At the end of the experiment, the average densitometry values of apoptotic cells in epididymis tubules were determined by TUNEL method; the protein and mRNA levels of VEGF and VEGFR2 were observed by immunohistochemistry, Western blot, and real time fluorescent quantitative PCR, respectively.
Results.
Compared with the control group, in each infected group, the average densitometry values of apoptotic cells in the epididymis tubules were significantly lower.
Compared with control group, protein and mRNA levels of VEGF and VEGFR2 in each infected group were obviously declined.
The correlations between protein and mRNA levels of VEGF and VEGFR2 were positively exhibited (r = 0.843, 0.869, p < 0.05).
Conclusions.
Arsenism affects the expressions of VEGF and VEGFR2 in the epididymis of rats and results in apoptosis of pathophysiology of male infertility.
American Psychological Association (APA)
Yan-Ping, Dai& Xiao-Qin, Gao& Xiao-Ping, Ma& Ying-Quan, Yue. 2017. Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134960
Modern Language Association (MLA)
Yan-Ping, Dai…[et al.]. Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1134960
American Medical Association (AMA)
Yan-Ping, Dai& Xiao-Qin, Gao& Xiao-Ping, Ma& Ying-Quan, Yue. Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1134960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1134960