Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a
Joint Authors
Hu, Dan-Ning
Gu, Ping
Zhang, Yingjie
Zhu, Yi
Sun, Hao
Zhu, Dongqing
Zhou, Jibo
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
To identify an effective method to prevent myopia progression by characterizing the regulation of matrix metalloproteinase- (MMP-) 2 expression and its secretion from scleral fibroblasts and retinal pigment epithelium (RPE) cells by miR-29a.
Methods.
The effects of miR-29a on the growth of scleral fibroblasts and RPE cells were assessed using the cell counting kit-8.
The changes in MMP-2 mRNA levels in scleral fibroblasts and RPE cells after transfection with miR-29a mimics or inhibitor were measured by quantitative PCR.
Enzyme-linked immunosorbent assays were used to determine the changes in MMP-2 secretion from scleral fibroblasts and RPE cells after transfection with miR-29a mimics or inhibitor.
Results.
The miR-29a mimics or inhibitor did not significantly alter the growth of scleral fibroblasts or RPE cells at 24, 48, or 72 hours after transfection.
MMP-2 mRNA levels were significantly decreased in scleral fibroblasts and RPE cells transfected with the miR-29a mimics.
The secretion of MMP-2 by scleral fibroblasts and RPE cells was significantly decreased in cells transfected with the miR-29a mimics.
Conclusions.
Suppression of scleral fibroblast and RPE cell expression and secretion of MMP-2 by miR-29a can be used as a therapeutic target for the prevention and treatment of myopia.
American Psychological Association (APA)
Zhang, Yingjie& Hu, Dan-Ning& Zhu, Yi& Sun, Hao& Gu, Ping& Zhu, Dongqing…[et al.]. 2017. Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1135006
Modern Language Association (MLA)
Zhang, Yingjie…[et al.]. Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1135006
American Medical Association (AMA)
Zhang, Yingjie& Hu, Dan-Ning& Zhu, Yi& Sun, Hao& Gu, Ping& Zhu, Dongqing…[et al.]. Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1135006
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135006