miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2PI3K Pathway
Joint Authors
Ma, Tengfei
Cai, Xiuqin
Wang, Zifeng
Huang, Li
Wang, Chang
Jiang, Songshan
Hua, Yunpeng
Liu, Quentin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Although expression of miR-200s is aberrant in liver fibrosis, its role in liver fibrogenesis still remains unknown.
Here, we investigated the role of miR-200c in the activation of human hepatic stellate cells (HSCs) and induction of liver fibrosis.
Methods.
We engineered human HSCs (LX2 cell line) to stably express miR-200c (LX2-200c) or empty vector control (LX2-nc).
Results.
miR-200c expression upregulated α-smooth muscle actin (SMA) and vimentin, enhanced HSCs growth and migration, increased expression of collagen type I (a main component of ECM) gene and secretion of epidermal growth factor (EGF), and upregulated the phosphorylation of Akt, a downstream effector of the PI3K pathway.
As a target of miR-200s and inhibitor of PI3K pathway, FOG2 protein expression was significantly suppressed in LX2-200c cells.
Moreover, LY294002, a highly selective inhibitor of PI3K, blocked phosphorylation of Akt and the effects of miR-200c.
Conclusions.
These data suggest that miR-200c activates HSCs in liver fibrosis possibly by downregulating FOG2 protein expression and upregulating PI3K/Akt signaling.
Autocrine activation of EGF signaling may also be a mechanism of miR-200c-mediated HSCs activation.
So miR-200c can be a potential marker for HSCs activation and liver fibrosis progression, as well as a potential target to attenuate liver fibrosis.
American Psychological Association (APA)
Ma, Tengfei& Cai, Xiuqin& Wang, Zifeng& Huang, Li& Wang, Chang& Jiang, Songshan…[et al.]. 2017. miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2PI3K Pathway. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135014
Modern Language Association (MLA)
Ma, Tengfei…[et al.]. miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2PI3K Pathway. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1135014
American Medical Association (AMA)
Ma, Tengfei& Cai, Xiuqin& Wang, Zifeng& Huang, Li& Wang, Chang& Jiang, Songshan…[et al.]. miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2PI3K Pathway. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135014