Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis
Joint Authors
Rodrigues, Ana M.
Canhão, Helena
Perpétuo, Inês P.
Caetano-Lopes, Joana
Campanilho-Marques, Raquel
Ponte, Cristina
Ainola, Mari
Fonseca, Joao Eurico
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Tumor necrosis factor (TNF) increases circulating osteoclast (OC) precursors numbers by promoting their proliferation and differentiation.
The aim of this study was to assess the effect of TNF inhibitors (TNFi) on the differentiation and activity of OC in rheumatoid arthritis (RA) patients.
Methods.
Seventeen RA patients treated with TNFi were analyzed at baseline and after a minimum follow-up period of 6 months.
Blood samples were collected to assess receptor activator of nuclear factor kappa-B ligand (RANKL) surface expression on circulating leukocytes and frequency and phenotype of monocyte subpopulations.
Quantification of serum levels of bone turnover markers, in vitro OC differentiation assays, and qRT-PCR for OC specific genes was performed.
Results.
After TNFi therapy, patients had reduced RANKL surface expression in B-lymphocytes and the frequency of circulating classical C D 14 b r i g h t CD16− monocytes was decreased.
Serum levels of sRANKL, sRANKL/OPG ratio, and CTX-I were reduced in RA patients after TNFi treatment.
Moreover, after exposure to TNFi, osteoclast differentiation and activity were decreased, as well as the expression of TRAF6 and cathepsin K.
Conclusion.
We propose that TNFi arrests bone loss and erosion, through two pathways: direct reduction of osteoclast precursor numbers and inhibition of intracellular signaling pathways acting through TRAF6.
American Psychological Association (APA)
Perpétuo, Inês P.& Caetano-Lopes, Joana& Rodrigues, Ana M.& Campanilho-Marques, Raquel& Ponte, Cristina& Canhão, Helena…[et al.]. 2017. Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135059
Modern Language Association (MLA)
Perpétuo, Inês P.…[et al.]. Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1135059
American Medical Association (AMA)
Perpétuo, Inês P.& Caetano-Lopes, Joana& Rodrigues, Ana M.& Campanilho-Marques, Raquel& Ponte, Cristina& Canhão, Helena…[et al.]. Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135059