Misaponin B Induces G2M Arrest, Cytokinesis Failure and Impairs Autophagy
Joint Authors
Sohn, Eun Jung
Jung, Ji-Hoon
Won, Gunho
Park, Ji Eon
Kim, Hyungjin
Lee, Hyo-Jung
Shim, Bum Sang
Kim, Sung-Hoon
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Saponins are a group of naturally occurring plant glycosides with the features of their strong foam-forming properties and multibiological effects such as antitumor activity.
Though Misaponin B, one of the triterpenoid saponins from Madhuca longifolia, is known to have spermicidal and antioxidant activity, the other biological activities have been never reported so far.
Thus, in the present study, the antitumor mechanism of Misaponin B was investigated in A549 and AsPC-1 cancer cells.
Misaponin B exerted significant cytotoxicity in A549, H460, SKOV3, and AsPC-1 cancer cells.
Among them, A549 and AsPC-1 cells were more susceptible to Misaponin B.
Misaponin B induced G2/M arrest and cytokinesis failure and increased the expression of LC3B and p62 with autophagic vacuoles and GFP-LC3 punctae in A549 and AsPC-1 cells.
Furthermore, Misaponin B suppressed autophagy flux in A549 cells transfected by GFP-mRFP-LC3 constructs by showing merged yellow color by autophagy flux assay.
Overall, our findings provide evidences that Misaponin B induces G2M arrest and impairs autophagy in A549 and AsPC-1 cells.
American Psychological Association (APA)
Won, Gunho& Jung, Ji-Hoon& Sohn, Eun Jung& Park, Ji Eon& Kim, Hyungjin& Lee, Hyo-Jung…[et al.]. 2020. Misaponin B Induces G2M Arrest, Cytokinesis Failure and Impairs Autophagy. BioMed Research International،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1135105
Modern Language Association (MLA)
Won, Gunho…[et al.]. Misaponin B Induces G2M Arrest, Cytokinesis Failure and Impairs Autophagy. BioMed Research International No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1135105
American Medical Association (AMA)
Won, Gunho& Jung, Ji-Hoon& Sohn, Eun Jung& Park, Ji Eon& Kim, Hyungjin& Lee, Hyo-Jung…[et al.]. Misaponin B Induces G2M Arrest, Cytokinesis Failure and Impairs Autophagy. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1135105
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135105