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Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response
Joint Authors
Li, Xiaomin
Zhuo, Luo
Chen, Xiaobing
Sun, Yan
Wang, Yanli
Shi, Yuanfeng
Bu, Lin
Xia, Wei
Han, Jiayan
Chen, Dongmei
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pyroptosis, an inflammatory form of programmed cell death, is the initiating event of sepsis and results in immune imbalance by releasing IL-1β and IL-18 in the early stages.
Studies show that enhancing autophagy via genetic manipulation can inhibit pyroptosis and prolong the survival of a sepsis animal model, indicating a possible therapeutic strategy against sepsis.
However, almost no study so far has achieved pyroptosis inhibition via pharmacological autophagy induction in a sepsis disease model.
To this end, we established an in vitro sepsis model by stimulating primary human umbilical vein endothelial cells (HUVECs) with lipopolysaccharide (LPS), and analyzed the effect of the autophagy agonist rapamycin (RAPA) on pyroptosis.
Phorbol 12-myristate 13-acetate- (PMA-) activated human THP-1 cells were used as the positive control.
LPS significantly increased the levels of the pyroptotic protein Gasdermin D (GSDMD), cysteinyl aspartate-specific proteinase 1 (caspase-1), secreted LDH, IL-1β, and IL-18.
RAPA treatment downregulated the above factors and enhanced autophagy in the LPS-stimulated HUVECs and THP-1 cells.
This study shows that RAPA abrogates LPS-mediated increase in IL-1β and IL-18 by inhibiting pyroptosis and enhancing autophagy.
American Psychological Association (APA)
Zhuo, Luo& Chen, Xiaobing& Sun, Yan& Wang, Yanli& Shi, Yuanfeng& Bu, Lin…[et al.]. 2020. Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135122
Modern Language Association (MLA)
Zhuo, Luo…[et al.]. Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1135122
American Medical Association (AMA)
Zhuo, Luo& Chen, Xiaobing& Sun, Yan& Wang, Yanli& Shi, Yuanfeng& Bu, Lin…[et al.]. Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1135122
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135122