The lncRNA H19 Promotes Cell Proliferation by Competitively Binding to miR-200a and Derepressing β-Catenin Expression in Colorectal Cancer
Joint Authors
Yang, Weiwei
Ning, Ning
Jin, Xiaoming
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
H19, a paternally imprinted noncoding RNA, has been found to be overexpressed in various cancers, including colorectal cancer (CRC), and may function as an oncogene.
However, the mechanism by which H19 regulates CRC progression remains poorly understood.
In this study, we aimed to assess H19 expression levels in CRC tissues, determine the effect of H19 on CRC proliferation, and explore the mechanism by which H19 regulates the proliferation of CRC.
We measured H19 expression using qRT-PCR and analysed the effects of H19 on colon cancer cell proliferation via cell growth curve, cell viability assay, and colony formation assays.
To elucidate the mechanism underlying these effects, we analysed the interactions between H19 and miRNAs and identified the target gene to which H19 and miRNA competitively bind using a series of molecular biological techniques.
H19 expression was upregulated in CRC tissues compared with adjacent noncancerous tissues.
H19 overexpression facilitated colon cancer cell proliferation, whereas H19 knockdown inhibited cell proliferation.
miR-200a bound to H19 and inhibited its expression, thereby decreasing CRC cell proliferation.
β-Catenin was identified as a target gene of miR-200a.
H19 regulated β-catenin expression and activity by competitively binding to miR-200a.
H19 promotes cell proliferation by competitively binding to miR-200a and derepressing β-catenin in CRC.
American Psychological Association (APA)
Yang, Weiwei& Ning, Ning& Jin, Xiaoming. 2017. The lncRNA H19 Promotes Cell Proliferation by Competitively Binding to miR-200a and Derepressing β-Catenin Expression in Colorectal Cancer. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135395
Modern Language Association (MLA)
Yang, Weiwei…[et al.]. The lncRNA H19 Promotes Cell Proliferation by Competitively Binding to miR-200a and Derepressing β-Catenin Expression in Colorectal Cancer. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1135395
American Medical Association (AMA)
Yang, Weiwei& Ning, Ning& Jin, Xiaoming. The lncRNA H19 Promotes Cell Proliferation by Competitively Binding to miR-200a and Derepressing β-Catenin Expression in Colorectal Cancer. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135395