Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB
Joint Authors
Rigane, E.
Dutoit, R.
Matthijs, S.
Brandt, N.
Flahaut, S.
Belghith, K. S.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium able to survive in diverse environments such as soil, plants, freshwater, and seawater.
P.
aeruginosa can be an opportunistic pathogen to humans when their immune system is deficient.
Its pathogenicity may be linked to the production of virulence factors.
We isolated P.
aeruginosa strain RBS from the saltern of Sfax in Tunisia.
In this study, we characterized the halotolerance, antibiotic susceptibility, and some virulence factors of strain RBS.
High NaCl concentrations inhibited growth and motility.
However, biofilm formation was enhanced to protect bacteria against salt stress.
Among the 18 antibiotics tested, quinolones and tetracycline showed a significant inhibitory effect on growth, motility, and biofilm formation of strain RBS.
β-Lactams, however, did not have any inhibitory effect on neither bacterial growth nor motility.
In some cases, resistance was due, in part, to biofilm formation.
We also showed that RBS produces two proteases, LasB and AprA, which have been shown to be implicated in host infection.
LasB was further characterized to study the role of metal ions in enzyme stability.
It possesses two distinct metal ion-binding sites coordinating a calcium and a zinc ion.
The effect of metal ion chelation was evaluated as well as substitutions of residues involved in metal ion binding.
Impairing metal ion binding of LasB led to a loss of activity and a sharp decrease of stability.
Our findings suggest that the binding of both metal ions is interdependent as the two metal ions’ binding sites are linked via a hydrogen bond network.
American Psychological Association (APA)
Rigane, E.& Dutoit, R.& Matthijs, S.& Brandt, N.& Flahaut, S.& Belghith, K. S.. 2020. Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1135451
Modern Language Association (MLA)
Rigane, E.…[et al.]. Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1135451
American Medical Association (AMA)
Rigane, E.& Dutoit, R.& Matthijs, S.& Brandt, N.& Flahaut, S.& Belghith, K. S.. Characterization of Putative Virulence Factors of Pseudomonas aeruginosa Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1135451
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135451