Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways
Joint Authors
Shen, Huangxuan
Yan, Jianhua
Long, Chongde
Liu, Huijun
Mao, Yuxiang
Xia, Bing
Kuang, Xielan
Huang, Hao
Ning, Jie
Ma, Xinqi
Zhang, Han
Wang, Renchun
Tang, Han
Du, Han
Zhang, Qingjiong
Zhang, Xinyu
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Posterior capsule opacification (PCO), a complication of extracapsular lens extraction surgery that causes visual impairment, is characterized by aberrant proliferation and epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs).
Curcumin, exerting inhibitive effects on cell proliferation and EMT in cancer, serves as a possible antidote towards PCO.
Methods.
Cellular proliferation of LECs after treatment of curcumin was measured with MTT assay and flow cytometry.
The transcriptional and expressional levels of proteins related to proliferation and EMT of LECs were quantified by western blotting and real-time PCR.
Results.
Curcumin was found to suppress the proliferation of LECs by inducing G2/M arrest via possible inhibition of cell cycle-related proteins including CDK1, cyclin B1, and CDC25C.
It had also inactivated proliferation pathways involving ERK1/2 and Akt pathways in LECs.
On the other hand, curcumin downregulated the EMT of LECs through blocking the TGF-β/Smad pathway and interfering Notch pathway which play important roles in PCO.
Conclusions.
This study shows that curcumin could suppress the proliferation and EMT in LECs, and it might be a potential therapeutic protection against visual loss induced by PCO.
American Psychological Association (APA)
Liu, Huijun& Mao, Yuxiang& Xia, Bing& Long, Chongde& Kuang, Xielan& Huang, Hao…[et al.]. 2020. Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1135461
Modern Language Association (MLA)
Liu, Huijun…[et al.]. Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1135461
American Medical Association (AMA)
Liu, Huijun& Mao, Yuxiang& Xia, Bing& Long, Chongde& Kuang, Xielan& Huang, Hao…[et al.]. Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1135461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135461