![](/images/graphics-bg.png)
Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury
Joint Authors
Li, Zu-Bing
Xing, Xin
Han, Shuang
Cheng, Gu
Ni, Yifeng
Li, Zhi
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Exosomes are extracellular membranous nanovesicles that mediate local and systemic cell-to-cell communication by transporting functional molecules, such as proteins, into target cells, thereby affecting the behavior of receptor cells.
Exosomes originating from adipose-derived mesenchymal stem cells (ADSCs) are considered a multipotent and abundant therapeutic tool for tissue injury.
To investigate ADSC-secreted exosomes and their potential function in tissue repair, we isolated exosomes from the supernatants of ADSCs via ultracentrifugation, characterized them via transmission electron microscopy, nanoparticle tracking analysis, and Western blot analysis.
Then, we determined their protein profile via proteomic analysis.
Results showed that extracellular vesicles, which have an average diameter of 116 nm, exhibit a cup-shaped morphology and express exosomal markers.
A total of 1,185 protein groups were identified in the exosomes.
Gene Ontology analysis indicated that exosomal proteins are mostly derived from cells mainly involved in protein binding.
Protein annotation via the Cluster of Orthologous Groups system indicated that most proteins were involved in general function prediction, posttranslational modification, protein turnover, and chaperoning.
Further, pathway analysis revealed that most of the proteins obtained participated in metabolic pathways, focal adhesion, regulation of the actin cytoskeleton, and microbial metabolism.
Some tissue repair-related signaling pathways were also discovered.
The identified molecules might serve as potential therapeutic targets for future studies.
American Psychological Association (APA)
Xing, Xin& Han, Shuang& Cheng, Gu& Ni, Yifeng& Li, Zhi& Li, Zu-Bing. 2020. Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135488
Modern Language Association (MLA)
Xing, Xin…[et al.]. Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1135488
American Medical Association (AMA)
Xing, Xin& Han, Shuang& Cheng, Gu& Ni, Yifeng& Li, Zhi& Li, Zu-Bing. Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135488