![](/images/graphics-bg.png)
Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3KAkt Signaling Pathway In Vitro
Joint Authors
Xie, Xiang-Cheng
Cao, Yizhi
Yang, Xiu
Xu, Qun-Hong
Wei, Wei
Wang, Ming
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Contrast-induced acute kidney injury (CI-AKI) is one of the main causes of iatrogenic acute kidney injury (AKI); however, therapeutic strategies for AKI remain limited.
This study aims to explore the effect of relaxin (RLX) on contrast-induced HK-2 apoptosis and its underlying mechanisms.
Methods.
Renal tubular epithelial cells (HK-2) were incubated either with or without ioversol, human H2 relaxin, and LY294002 (the inhibitor of the PI3K/Akt signal pathway).
Cell viability was evaluated with a CCK-8 assay.
Apoptotic morphologic alterations were observed using the Hoechst 33342 staining method.
Apoptosis was detected with Annexin V staining.
Western blot analysis was employed to measure the expression of pAkt (S473), Akt, cleaved caspase-3, Bcl-2, Bax, and actin proteins.
Results.
Ioversol reduced the viability of HK-2 cells.
Western blotting results revealed decreased expression of phosphorylated Akt in cells treated with ioversol.
The activities of caspase-3 and Bax protein increased, while the expression of Bcl-2 protein decreased.
As a result, the Bax/Bcl-2 ratio increased after treatment with ioversol.
These effects were reversed when HK-2 cells were cotreated with RLX.
However, with preadministration of PI3K/Akt pathway inhibitor LY294002, the effect of RLX was blocked.
Conclusion.
Our study demonstrates that relaxin attenuates ioversol induced cell apoptosis via activation of the PI3K/Akt signaling pathway, suggesting that RLX might play a protective role in the treatment of CI-AKI.
American Psychological Association (APA)
Xie, Xiang-Cheng& Cao, Yizhi& Yang, Xiu& Xu, Qun-Hong& Wei, Wei& Wang, Ming. 2017. Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3KAkt Signaling Pathway In Vitro. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1135505
Modern Language Association (MLA)
Xie, Xiang-Cheng…[et al.]. Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3KAkt Signaling Pathway In Vitro. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1135505
American Medical Association (AMA)
Xie, Xiang-Cheng& Cao, Yizhi& Yang, Xiu& Xu, Qun-Hong& Wei, Wei& Wang, Ming. Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3KAkt Signaling Pathway In Vitro. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1135505
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135505