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miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1
Joint Authors
Sun, Yi
Chen, Guanghua
Song, Chengchao
Ma, Xinnan
Fu, Yutuo
Yan, Jinglong
Feng, Chao
Wang, Xin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible.
Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment.
In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice.
Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro.
The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay.
Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1).
Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs.
Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases.
American Psychological Association (APA)
Sun, Yi& Wang, Xin& Chen, Guanghua& Song, Chengchao& Ma, Xinnan& Fu, Yutuo…[et al.]. 2020. miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135533
Modern Language Association (MLA)
Sun, Yi…[et al.]. miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1135533
American Medical Association (AMA)
Sun, Yi& Wang, Xin& Chen, Guanghua& Song, Chengchao& Ma, Xinnan& Fu, Yutuo…[et al.]. miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135533