Identification of Key Modules, Hub Genes, and Noncoding RNAs in Chronic Rhinosinusitis with Nasal Polyps by Weighted Gene Coexpression Network Analysis
Joint Authors
Zhou, Xuanchen
Zhen, Xiaoyue
Liu, Yiqing
Cui, Zhaoyang
Yue, Zhiyong
Xu, Anting
Han, Jie
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-25
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease with relatively easy recurrence.
However, the precise molecular mechanisms of this disease are poorly known.
Based on gene sequencing data obtained from the Gene Expression Omnibus (GEO) database, we constructed coexpression networks by weighted gene coexpression network analysis (WGCNA).
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed by the Database for Annotation, Visualization, and Integrated Discovery (DAVID).
The core gene of pathogenesis, CRSwNP, was screened by protein-protein interaction data (PPI) from the HPRD database.
Unsupervised clustering was applied to screen hub genes related to the phenotype of CRSwNP.
Blue and turquoise modules were found to be most significantly related to the pathogenicity of CRSwNP.
Functional enrichment analysis showed that cell proliferation in the blue modules, the apoptotic process in the turquoise module, and the cancer pathway in both modules were mostly significantly correlated with the development of CRSwNP.
The noncoding RNAs (long noncoding RNA and microRNA) and the top 10 core genes in each module were found to be associated with the pathogenesis of CRSwNP.
A total of nine hub genes were identified to be related to the CRSwNP phenotype.
By qRT-PCR analysis, AKT1, CDH1, PIK3R1, CBL, LRP1, MALAT1, and XIST were proven to be associated with the pathogenesis of CRSwNP.
AGR2, FAM3D, PIP, DSE, and TMC were identified to be related to the CRSwNP phenotype.
Further exploration of these genes will reveal more important information about the mechanisms of CRSwNP.
American Psychological Association (APA)
Zhou, Xuanchen& Zhen, Xiaoyue& Liu, Yiqing& Cui, Zhaoyang& Yue, Zhiyong& Xu, Anting…[et al.]. 2020. Identification of Key Modules, Hub Genes, and Noncoding RNAs in Chronic Rhinosinusitis with Nasal Polyps by Weighted Gene Coexpression Network Analysis. BioMed Research International،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1135539
Modern Language Association (MLA)
Zhou, Xuanchen…[et al.]. Identification of Key Modules, Hub Genes, and Noncoding RNAs in Chronic Rhinosinusitis with Nasal Polyps by Weighted Gene Coexpression Network Analysis. BioMed Research International No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1135539
American Medical Association (AMA)
Zhou, Xuanchen& Zhen, Xiaoyue& Liu, Yiqing& Cui, Zhaoyang& Yue, Zhiyong& Xu, Anting…[et al.]. Identification of Key Modules, Hub Genes, and Noncoding RNAs in Chronic Rhinosinusitis with Nasal Polyps by Weighted Gene Coexpression Network Analysis. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1135539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135539