Transient Receptor Potential Melastatin 2 Negatively Regulates LPS-ATP-Induced Caspase-1-Dependent Pyroptosis of Bone Marrow-Derived Macrophage by Modulating ROS Production
Joint Authors
Wang, Haihong
Qian, Xiaowei
Zhou, Xinyi
Li, Hui
Wang, Yan
Ma, Liang
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Pyroptosis, a new form of cell death, which has special morphological characteristics, depends on caspase-1 activation and occupies an important role in inflammatory immune diseases and ischemia-reperfusion injury.
ROS is a common activator of NLR/caspase-1.
Transient receptor potential melastatin 2 (TRPM2), a selective cation channel, is involved in inflammatory regulation.
This study was designed to explore the role of TRPM2 in activating caspase-1 and caspase-1-dependent pyroptosis of mouse BMDMs.
Methods.
BMDMs isolated from WT and TRPM2−/− mice were treated with LPS and ATP, along with ROS inhibitor (NAC and DPI), caspase-1 inhibitor (Z-YVAD), or not.
The activation of caspase-1 was measured by western blot.
EtBr and EthD-2 staining were used to assess the incidence of pyroptosis.
Results.
Compared with WT, the activated caspase-1-P10 was higher and the percentage of EtBr positive cells was also increased in TRPM2−/− group, which were both inhibited by Z-YVAD, NAC, or DPI.
ASC oligomerization was increased in TRPM2−/− group.
Conclusion.
Deletion of TRPM2 can enhance the activation of caspase-1 and pyroptosis, which may be via modulating ROS production, suggesting that TRPM2 plays a critical role in immune adjustment.
American Psychological Association (APA)
Wang, Haihong& Zhou, Xinyi& Li, Hui& Qian, Xiaowei& Wang, Yan& Ma, Liang. 2017. Transient Receptor Potential Melastatin 2 Negatively Regulates LPS-ATP-Induced Caspase-1-Dependent Pyroptosis of Bone Marrow-Derived Macrophage by Modulating ROS Production. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135603
Modern Language Association (MLA)
Wang, Haihong…[et al.]. Transient Receptor Potential Melastatin 2 Negatively Regulates LPS-ATP-Induced Caspase-1-Dependent Pyroptosis of Bone Marrow-Derived Macrophage by Modulating ROS Production. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1135603
American Medical Association (AMA)
Wang, Haihong& Zhou, Xinyi& Li, Hui& Qian, Xiaowei& Wang, Yan& Ma, Liang. Transient Receptor Potential Melastatin 2 Negatively Regulates LPS-ATP-Induced Caspase-1-Dependent Pyroptosis of Bone Marrow-Derived Macrophage by Modulating ROS Production. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1135603
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135603