miR-331-3p Inhibits Inflammatory Response after Intracerebral Hemorrhage by Directly Targeting NLRP6
Joint Authors
Hu, Yang
Tang, Zhouping
Nie, Hao
Li, Qi
Guo, Wenliang
Wang, Wenzhi
Yang, Qingwu
Dong, Qiang
Tang, Yuping
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
The mechanism of inflammatory reaction after intracerebral hemorrhage remains unclear, which to some extent restrains the therapeutic development of hemorrhagic stroke.
The present study attempts to verify whether NLRP6 plays an important role in inflammatory reaction after intracerebral hemorrhage and identify the critical microRNA during the process.
Methods.
Suitable simulated cerebral hemorrhage environments were established in vitro and in vivo.
BV2 cells were treated with hemin to induce cell damage.
Collagenase was used to establish a model of mouse cerebral hemorrhage.
The relationship among NLRP6, miR-331-3p, and the corresponding inflammatory expression was closely observed during this process.
Techniques, such as western blot, real-time quantitative PCR, immunofluorescence, and immunocytochemistry, were used to detect the expression of relative genes and molecules in the in vitro and in vivo models.
Results.
Downregulated miR-331-3p increased the expression of NLRP6 and alleviated the expression of TNF-α and IL-6.
The neurological function recovery of mice was promoted after intracerebral hemorrhage.
Conclusion.
miR-331-3p regulated the inflammatory response after cerebral hemorrhage by negatively regulating the expression of NLRP6.
American Psychological Association (APA)
Nie, Hao& Hu, Yang& Guo, Wenliang& Wang, Wenzhi& Yang, Qingwu& Dong, Qiang…[et al.]. 2020. miR-331-3p Inhibits Inflammatory Response after Intracerebral Hemorrhage by Directly Targeting NLRP6. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1135604
Modern Language Association (MLA)
Nie, Hao…[et al.]. miR-331-3p Inhibits Inflammatory Response after Intracerebral Hemorrhage by Directly Targeting NLRP6. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1135604
American Medical Association (AMA)
Nie, Hao& Hu, Yang& Guo, Wenliang& Wang, Wenzhi& Yang, Qingwu& Dong, Qiang…[et al.]. miR-331-3p Inhibits Inflammatory Response after Intracerebral Hemorrhage by Directly Targeting NLRP6. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1135604
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135604