The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1

Abstract EN

Borealin is a key component of chromosomal passenger complex, which is vital in cytokinesis.

IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis.

The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear.

Here, we used mass spectrometry and endogenous coimmunoprecipitation experiments to investigate the interaction between IQGAP1 and Borealin.

Results of the current study showed that Borealin interacted directly with IQGAP1 both in vitro and in vivo.

Knockdown of IQGAP1 resulted in an abnormal location of Borealin in the midbody.

Knocking down Borealin alone, IQGAP1 alone, or Borealin and IQGAP1 at the same time inhibited the completion of cytokinesis and formed multinucleated cells.

Our results indicated that IQGAP1 interacts with Borealin during cytokinesis, and the correct localization of Borealin in the midbody during cytokinesis is determined by IQGAP1, and IQGAP1 may play an important role in regulating Borealin function in cytokinesis.