Human Uterine Decidual NK Cells in Women with a History of Early Pregnancy Enhance Angiogenesis and Trophoblast Invasion

Abstract EN

Objective.

The present study aimed to identify changes in decidual natural killer (dNK) cells and related cytokines in women who have undergone induced abortions (IAs).

The effects of dNK cells on subsequent pregnancies remain unknown.

Accordingly, we sought to investigate whether a history of early pregnancy can change dNK cells and facilitate their role in the regulation of angiogenesis and trophoblast invasion.

Materials and Methods.

dNK cells were obtained from primiparous women who had undergone IA(s) prior to this study and primiparous women who had never been pregnant before this IA (control).

Real-time polymerase chain reaction (PCR) was used to measure the mRNA levels of IFN-γ, IP-10, VEGF, and PLGF in dNK cells.

The levels of these cytokines were quantified using the enzyme-linked immunosorbent assay.

HUVEC and HTR-8/SVneo cells were used to evaluate the angiogenesis, migration, and invasion activities influenced by dNK cells.

Results.

In dNK cells, the mRNA level of IFN-γ was higher in the control group than that in the IA group.

The secretion of IP-10 and VEGF was higher in the IA group compared to the control group.

After coculturing with the dNK supernatant, the HTR-8/SVneo cells exhibited better invasiveness and migration in the IA group than those in the control group.

Angiogenesis assay demonstrated that dNK cells from IA group might help HUVEC attain better tube formation ability.

Conclusion.

The findings of this study suggest that a history of early pregnancy has an impact on dNK cells.

These trained dNK cells can regulate angiogenesis and trophoblast invasion and migration by promoting the production of certain cytokines.