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Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in dbdb Mice
Joint Authors
Wang, Fan
Zhang, Chao
Dai, Linna
Zhang, Yulu
Wang, Yongxue
Hao, Yongwei
Ji, Shenglu
Xu, Zhihao
Han, Na
Chen, Hongli
Zhang, Qiqing
Nan, Wenbin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Numerous studies have reported that autophagy plays an important role in chronic wound healing, and enhancement of autophagic activity impairs cutaneous wound healing.
The autophagy inhibitor Bafilomycin A1 (Baf A1) inhibits autophagy by preventing the formation of autophagosomes.
This study aimed at elucidating the effect of Bafilomycin A1 on chronic refractory wound healing in diabetic mice.
A total of 40 diabetic (db/db) mice and 20 nondiabetic (db/m) mice were used in this study.
Full-thickness skin defects were generated in the db/db mice models, which were then divided into the following two groups: the nontreated (db/db group) and Baf A1-treated groups (Baf A1 group).
The same skin defects were generated in db/m mice (db/m group) to serve as a control.
We demonstrated that Baf A1 treatment significantly accelerated wound healing in db/db mice and exerted good healing effects.
Moreover, Baf A1 inhibited autophagy in the newly generated epidermis and had minor effects on metabolism in db/db mice.
PCNA expression, as detected by immunohistochemistry, and collagen thickness, as detected by Masson’s trichrome staining on the 14th day, were higher in the db/m and Baf A1 groups than in the db/db group.
In addition, the expression of the proinflammatory cytokine TNF-α in the db/m and Baf A1 groups increased significantly on day 6, and the expression of the anti-inflammatory cytokine IL-10 also increased significantly on day 9.
However, there were no significant changes in the expression levels of TNF-α and IL-10 in the db/db group.
Therefore, Baf A1 may accelerate diabetic chronic refractory wound healing by promoting cell proliferation, collagen production, and regulating the inflammatory balance.
American Psychological Association (APA)
Wang, Fan& Zhang, Chao& Dai, Linna& Zhang, Yulu& Wang, Yongxue& Hao, Yongwei…[et al.]. 2020. Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in dbdb Mice. BioMed Research International،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135687
Modern Language Association (MLA)
Wang, Fan…[et al.]. Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in dbdb Mice. BioMed Research International No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1135687
American Medical Association (AMA)
Wang, Fan& Zhang, Chao& Dai, Linna& Zhang, Yulu& Wang, Yongxue& Hao, Yongwei…[et al.]. Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in dbdb Mice. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1135687
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135687