Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types
Joint Authors
Al-Bahlani, Shadia
Al-Dhahli, Buthaina
Al-Adawi, Kawther
Al-Nabhani, Abdurahman
Al-Kindi, Mohamed
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Breast cancer (BC) is the most common cause of cancer-related death worldwide.
Although platinum-based drugs (PBDs) are effective anticancer agents, responsive patients eventually become resistant.
While resistance of some cancers to PBDs has been explored, the cellular responses of BC cells are not studied yet.
Therefore, we aim to assess the differential effects of PBDs on BC ultrastructure.
Three representative cells were treated with different concentrations and timing of Cisplatin, Carboplatin, and Oxaliplatin.
Changes on cell surface and ultrastructure were detected by scanning (SEM) and transmission electron microscope (TEM).
In SEM, control cells were semiflattened containing microvilli with extending lamellipodia while treated ones were round with irregular surface and several pores, indicating drug entry.
Prolonged treatment resembled distinct apoptotic features such as shrinkage, membrane blebs, and narrowing of lamellipodia with blunt microvilli.
TEM detected PBDs’ deposits that scattered among cellular organelles inducing structural distortion, lumen swelling, chromatin condensation, and nuclear fragmentation.
Deposits were attracted to fat droplets, explained by drug hydrophobic properties, while later they were located close to cell membrane, suggesting drug efflux.
Phagosomes with destructed organelles and deposits were detected as defending mechanism.
Understanding BC cells response to PBDs might provide new insight for an effective treatment.
American Psychological Association (APA)
Al-Bahlani, Shadia& Al-Dhahli, Buthaina& Al-Adawi, Kawther& Al-Nabhani, Abdurahman& Al-Kindi, Mohamed. 2017. Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types. BioMed Research International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1135817
Modern Language Association (MLA)
Al-Bahlani, Shadia…[et al.]. Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types. BioMed Research International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1135817
American Medical Association (AMA)
Al-Bahlani, Shadia& Al-Dhahli, Buthaina& Al-Adawi, Kawther& Al-Nabhani, Abdurahman& Al-Kindi, Mohamed. Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1135817
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1135817