Research on the Effect of Pediococcus pentosaceus on Salmonella enteritidis-Infected Chicken

Joint Authors

Lan, Dan
Xun, XinYu
Hu, YaoDong
Li, NianZhen
Yang, ChaoWu
Jiang, XiaoSong
Liu, YiPing

Source

BioMed Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-10-12

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

Salmonella enteritidis can cause significant morbidity and mortality in humans and economic loss in the animal industry.

Improving the innate immunity is an effective method to prevent S.

enteritidis infection.

Pediococcus pentosaceus is a Gram-positive coccus which had probiotics properties.

Numerous previously published studies reported that probiotics were beneficial to gut microbiota by changing the intestinal flora structure and inhibiting the harmful microbial growth to enhance the innate immunity.

We investigated the immunological effects of P.

pentosaceus on Salmonella-infected chickens by the following experiment.

A total of 120 broilers from AA line were fed and divided into 2 groups (treated and control groups) for the experiment from day 1.

The control group was fed with the basic diet, while the treated group was fed with the basic diet adding P.

pentosaceus microcapsule with the bacterial concentration of 1 g/kg in the feed and bacterial counts 2.5×109 CFU/g.

All the birds were given with 0.5 ml of S.

enteritidis bacterial suspension (109 CFU/ml) through oral cavity at day 9.

The number of dead birds was recorded and used in the analysis.

The bacterial culture method and quantitative real-time PCR analysis were used to evaluate the effects of P.

pentosaceus on chickens infected with S.

enteritidis and to ascertain the mechanism of the effect.

The results showed that the P.

pentosaceus could restrain the pathogenicity of S.

enteritidis and reduce the death rate from 44.4% to 23.3%.

The flora in the caecum exhibited “rising-declining” trends, and the gene (TLR4, MyD88, TRAF6 NF-κB, IFN-β, TNF-a, IL6, and IL8) expression pattern was different between the experimental and control group.

P.

pentosaceus as a probiotic may competitively inhibit the growth of S.

enteritidis and control the inflammatory response through regulating the gene expression which involved in the toll-like receptor pathway and inflammation pathway.

American Psychological Association (APA)

Lan, Dan& Xun, XinYu& Hu, YaoDong& Li, NianZhen& Yang, ChaoWu& Jiang, XiaoSong…[et al.]. 2020. Research on the Effect of Pediococcus pentosaceus on Salmonella enteritidis-Infected Chicken. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135859

Modern Language Association (MLA)

Lan, Dan…[et al.]. Research on the Effect of Pediococcus pentosaceus on Salmonella enteritidis-Infected Chicken. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1135859

American Medical Association (AMA)

Lan, Dan& Xun, XinYu& Hu, YaoDong& Li, NianZhen& Yang, ChaoWu& Jiang, XiaoSong…[et al.]. Research on the Effect of Pediococcus pentosaceus on Salmonella enteritidis-Infected Chicken. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1135859

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1135859