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lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation
Joint Authors
Liu, Jinlong
Chen, Kai
Zhang, Zhuqing
Yu, Aijun
Zhang, Xuejun
Li, Jian
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
DLGAP1-AS2 has been characterized as an oncogenic lncRNA in glioma.
Our preliminary microarray analysis revealed the altered expression of DLGAP1-AS2 in hepatocellular carcinoma (HCC), but the role of DLGAP1-AS2 in HCC remains unknown.
Method.
Expression of DLGAP1-AS2 and miR-154-5p in paired HCC and nontumor tissues from 62 HCC patients was determined by RT-qPCR.
The 62 HCC patients were followed up for 5 years to analyze the prognostic value of DLGAP1-AS2 for HCC.
DLGAP1-AS2 knockdown and miR-154-5p overexpression was achieved in HCC cells to study the relationship between them.
Methylation of miR-154-5p was analyzed by methylation-specific PCR.
Cell proliferation was analyzed by CCK-8 assay.
Results.
DLGAP1-AS2 was upregulated in HCC and predicted poor survival.
miR-154-5p was downregulated in HCC and inversely correlated with DLGAP1-AS2.
In HCC cells, DLGAP1-AS2 knockdown resulted in the upregulation of miR-154-5p expression and decreased methylation of miR-154-5p gene.
Transwell assay showed that DLGAP1-AS2 knockdown and miR-154-5p overexpression inhibited cell invasion and migration, and the combination of LGAP1-AS2 knockdown and miR-154-5p overexpression showed stronger effects.
Conclusion.
DLGAP1-AS2 knockdown may inhibit HCC cell migration and invasion by regulating miR-154-5p methylation.
American Psychological Association (APA)
Chen, Kai& Zhang, Zhuqing& Yu, Aijun& Li, Jian& Liu, Jinlong& Zhang, Xuejun. 2020. lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation. BioMed Research International،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1136003
Modern Language Association (MLA)
Chen, Kai…[et al.]. lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation. BioMed Research International No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1136003
American Medical Association (AMA)
Chen, Kai& Zhang, Zhuqing& Yu, Aijun& Li, Jian& Liu, Jinlong& Zhang, Xuejun. lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1136003
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136003