Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells

Joint Authors

Xin, Haiming
Zhu, Jinhong
Miao, Hongcheng
Gong, Zhenyu
Jiang, Xiaochen
Feng, Xiaoyan
Tong, Yalin

Source

BioMed Research International

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-03-14

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Our previous report revealed that immature dendritic cells (imDCs) with adenovirus-mediated CCR7 overexpression acquired an enhanced migratory ability but also exhibited the lower immune tolerance observed in more mature cells.

In the present study, we aimed to investigate whether BTLA overexpression was sufficient to preserve immune tolerance in imDCs with exogenous CCR7 overexpression.

Scanning electron microscopy and surface antigens analysis revealed that BTLA overexpression suppressed DC maturation, an effect further potentiated in CCR7 and BTLA cooverexpressing cells.

Correspondingly, in vitro chemotaxis assays and mixed lymphocyte reactions demonstrated increased migratory potential and immune tolerance in CCR7 and BTLA coexpressing cells.

Furthermore, CCR7 and BTLA cooverexpressed imDCs suppressed IFN-γ and IL-17 expression and promoted IL-4 and TGF-beta expression of lymphocyte, indicating an increase of T helper 2 (Th2) regulatory T cell (Treg).

Thus, these data indicate that CCR7 and BTLA cooverexpression imparts an intermediate immune phenotype in imDCs when compared to that in CCR7- or BTLA-expressing counterparts that show a more immunocompetent or immunotolerant phenotype, respectively.

All these results indicated that adenovirus-mediated CCR7 and BTLA overexpression could enhance immune tolerance and migration of imDCs.

Our study provides a basis for further studies on imDCs in immune tolerance, with the goal of developing effective cellular immunotherapies for transplant recipients.

American Psychological Association (APA)

Xin, Haiming& Zhu, Jinhong& Miao, Hongcheng& Gong, Zhenyu& Jiang, Xiaochen& Feng, Xiaoyan…[et al.]. 2017. Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1136049

Modern Language Association (MLA)

Xin, Haiming…[et al.]. Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1136049

American Medical Association (AMA)

Xin, Haiming& Zhu, Jinhong& Miao, Hongcheng& Gong, Zhenyu& Jiang, Xiaochen& Feng, Xiaoyan…[et al.]. Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1136049

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1136049