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Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs
Joint Authors
Elsaadany, Mostafa
Harris, Matthew
Yildirim-Ayan, Eda
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
It is crucial to replicate the micromechanical milieu of native tissues to achieve efficacious tissue engineering and regenerative therapy.
In this study, we introduced an innovative loading platform, EQUicycler, that utilizes a simple, yet effective, and well-controlled mechanism to apply physiologically relevant homogenous mechanical equiaxial strain on three-dimensional cell-embedded tissue scaffolds.
The design of EQUicycler ensured elimination of gripping effects through the use of biologically compatible silicone posts for direct transfer of the mechanical load to the scaffolds.
Finite Element Modeling (FEM) was created to understand and to quantify how much applied global strain was transferred from the loading mechanism to the tissue constructs.
In vitro studies were conducted on various cell lines associated with tissues exposed to equiaxial mechanical loading in their native environment.
In vitro results demonstrated that EQUicycler was effective in maintaining and promoting the viability of different musculoskeletal cell lines and upregulating early differentiation of osteoprogenitor cells.
By utilizing EQUicycler, collagen fibers of the constructs were actively remodeled.
Residing cells within the collagen construct elongated and aligned with strain direction upon mechanical loading.
EQUicycler can provide an efficient and cost-effective tool to conduct mechanistic studies for tissue engineered constructs designed for tissue systems under mechanical loading in vivo.
American Psychological Association (APA)
Elsaadany, Mostafa& Harris, Matthew& Yildirim-Ayan, Eda. 2017. Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs. BioMed Research International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1136132
Modern Language Association (MLA)
Elsaadany, Mostafa…[et al.]. Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs. BioMed Research International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1136132
American Medical Association (AMA)
Elsaadany, Mostafa& Harris, Matthew& Yildirim-Ayan, Eda. Design and Validation of Equiaxial Mechanical Strain Platform, EQUicycler, for 3D Tissue Engineered Constructs. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1136132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136132