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Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia
Joint Authors
Cheng, Sokleaph
Pheng, Sok Heng
Heng, Seiha
Bañuls, Anne-Laure
Mao, Tan Eang
Kerléguer, Alexandra
Marks, Guy B.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The Loopamp™ MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate.
This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients.
499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory.
Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert® MTB/RIF.
At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]).
At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample.
No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites.
In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients.
In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay.
American Psychological Association (APA)
Cheng, Sokleaph& Pheng, Sok Heng& Heng, Seiha& Marks, Guy B.& Bañuls, Anne-Laure& Mao, Tan Eang…[et al.]. 2020. Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia. BioMed Research International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136233
Modern Language Association (MLA)
Cheng, Sokleaph…[et al.]. Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia. BioMed Research International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1136233
American Medical Association (AMA)
Cheng, Sokleaph& Pheng, Sok Heng& Heng, Seiha& Marks, Guy B.& Bañuls, Anne-Laure& Mao, Tan Eang…[et al.]. Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1136233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136233