![](/images/graphics-bg.png)
Bacterial Diversity in Feline Conjunctiva Based on 16S rRNA Gene Sequence Analysis: A Pilot Study
Joint Authors
Bania, Jacek
Bierowiec, Karolina
Płoneczka-Janeczko, Katarzyna
Kiełbowicz, Maciej
Kiełbowicz, Zdzisław
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-27
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Changes in the microbial populations in the conjunctival sacs of animals have traditionally been evaluated using conventional microbiology techniques.
The goal of this study was to examine the suitability of a methodology which may reveal a previously unknown microbiome inhabiting feline conjunctival membranes.
In the present study, we determined the microbial diversity in feline conjunctivas based on 16S rRNA gene sequence analysis.
Five taxa not described earlier in veterinary ophthalmology (i.e., Staphylococcus caprae, Staphylococcus succinus, Propionibacterium acnes, Psychrobacter faecalis, and Bacillus subtilis) were identified in feline conjunctivas with a high similarity (99-100%).
The study demonstrates that the feline conjunctival sacs are inhabited by much more rich and diverse microbial communities than previously thought using culture-based methods.
From the clinical perspective, this could suggest that other laboratory procedures (e.g., extended incubation time in the case of Actinobacteria, formerly order Actinomycetales) or a new tool like culture-independent approaches (next-generation DNA sequencing) should be taken into account.
American Psychological Association (APA)
Płoneczka-Janeczko, Katarzyna& Bania, Jacek& Bierowiec, Karolina& Kiełbowicz, Maciej& Kiełbowicz, Zdzisław. 2017. Bacterial Diversity in Feline Conjunctiva Based on 16S rRNA Gene Sequence Analysis: A Pilot Study. BioMed Research International،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1136271
Modern Language Association (MLA)
Płoneczka-Janeczko, Katarzyna…[et al.]. Bacterial Diversity in Feline Conjunctiva Based on 16S rRNA Gene Sequence Analysis: A Pilot Study. BioMed Research International No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1136271
American Medical Association (AMA)
Płoneczka-Janeczko, Katarzyna& Bania, Jacek& Bierowiec, Karolina& Kiełbowicz, Maciej& Kiełbowicz, Zdzisław. Bacterial Diversity in Feline Conjunctiva Based on 16S rRNA Gene Sequence Analysis: A Pilot Study. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1136271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136271