Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways
Joint Authors
Xie, Conghua
Wang, Xinghuan
Hu, Qingxuan
Wang, Gang
Peng, Jianping
Qian, Guofeng
Jiang, Wei
Xiao, Yu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-25
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Bladder cancer (BCa) is one of the most common tumors, but its underlying mechanism has not been fully clarified.
Our transcriptome analysis suggested a close link of Sirtuins, Peroxisome Proliferator-Activated Receptor (PPAR), cell cycle regulation, reactive oxygen species (ROS) metabolism, and Forkhead Box Class O (FOXO) signaling pathway in BCa.
SIRT1 is a key member of Sirtuins, playing important roles in aging and energy metabolism, which has been reported to be involved in various metabolic diseases and tumors.
We observed that SIRT1 was upregulated in BCa tissues at both mRNA and protein levels.
By establishing a SIRT1-knockdown BCa cell model, our results suggested that proliferation and viability were suppressed.
Moreover, migration rate was inhibited as well, possibly via reduction of epithelial-mesenchymal transition (EMT).
In addition, cell cycle arrest was significantly induced, consisting with strongly decreased proteins involved (CDK2/4/6).
Furthermore, ROS production was slightly reduced, accompanied by increasing of antioxidant enzymes and total/acetylated FOXO3a.
Consistently with our Path-net analysis, we observed no significant alteration of apoptosis in the SIRT1-knockdown BCa cells.
Taken together, our results suggested that SIRT1 deficiency in BCa cells could suppress cell viability by activating antioxidant response and inducing cell cycle arrest possibly via FOXO3a-related pathways.
American Psychological Association (APA)
Hu, Qingxuan& Wang, Gang& Peng, Jianping& Qian, Guofeng& Jiang, Wei& Xie, Conghua…[et al.]. 2017. Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways. BioMed Research International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1136377
Modern Language Association (MLA)
Hu, Qingxuan…[et al.]. Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways. BioMed Research International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1136377
American Medical Association (AMA)
Hu, Qingxuan& Wang, Gang& Peng, Jianping& Qian, Guofeng& Jiang, Wei& Xie, Conghua…[et al.]. Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1136377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136377