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Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR
Joint Authors
Chaves, Mariana H.
Carneiro, Fabiana Pirani
Darós, Andersen Charles
Darós, Adriana Cysneiro Milhomem
de Castro, Tércia Maria Mendes Lousa
de Vasconcelos Carneiro, Marcos
Fidelis, Cecília Ramos
Vilioni, Mariane Vieira
da Costa Matsunaga, Michelle Egídio
Sidou, Jéssica Meneses Othon
Pereira, Lívia Custódio
de Resende, Ceres Nunes
de Castro Moreira dos Santos, Agenor
Ferreira, Vânia Moraes
Motoyama, Andrea Barretto
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described.
The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum detected by multiplex PCR.
Methods.
Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit.
Results.
Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0).
Multiple pathogens were observed in 12.8% of samples.
Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common.
In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%.
Conclusion.
Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, or Trichomonas vaginalis.
A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.
American Psychological Association (APA)
Carneiro, Fabiana Pirani& Darós, Andersen Charles& Darós, Adriana Cysneiro Milhomem& de Castro, Tércia Maria Mendes Lousa& de Vasconcelos Carneiro, Marcos& Fidelis, Cecília Ramos…[et al.]. 2020. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1136443
Modern Language Association (MLA)
Carneiro, Fabiana Pirani…[et al.]. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1136443
American Medical Association (AMA)
Carneiro, Fabiana Pirani& Darós, Andersen Charles& Darós, Adriana Cysneiro Milhomem& de Castro, Tércia Maria Mendes Lousa& de Vasconcelos Carneiro, Marcos& Fidelis, Cecília Ramos…[et al.]. Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1136443
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136443