Pathological study and genetic diagnosis of vinblastine effects on dna of ovary and testes in mouse

Other Title(s)

دراسة جزيئية-نسيجية-مرضية في تأثيرالفنبلاستين في دنا المبيض و الخصى في الفئران البيضاء

Joint Authors

Hammadi, Taghrid Jabbar
al-Amiri, Muna Sajit Hashim
Mahmud, Dhikra Abd Allah
Muhammad, Suad Abd al-Karim
Nasir, Rabab Abd al-Amir

Source

مجلة ديالى للعلوم الزراعية

Publisher

University of Diyala College of Agriculture

Publication Date

2018-12-31

Country of Publication

Iraq

No. of Pages

12

Main Subjects

Biology

Topics

• Nucleic acids
• Leukocytes
• Esterases

English Abstract

The present study was designed in order to identify the effects of vinblastine on ovary and testes and then on fertilization capacity and pregnancy outcome in white mice, fifty mixed mice were used, 10 treated male were mated with 10 untreated female, 10 untreated male were mated with 10 treated female, and (10) mixed mice as control.

Results of male test showed decline in sperm count with spermatocytes' morphological deformity.

The results of molecular technique applied on testicular section and ovarian section, showed that green signals, indicating the damage on TK (11qE2)/XY gene on chromosome of sperm and ova, red signal indicate intact gene yellowish signals indicated translocation on the same gene (mutation).

Pathological findings showed marked loss of spermatogenesis (Azoospermia) with damage in testes' tissues; fibrosis of ovarian stroma with degenerative changes on ovary and evidence of early embryonic death with ence or low fertility

Data Type

Conference Papers

Record ID

BIM-1136492

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