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Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMPPKA Pathway
Joint Authors
Zhang, Liang
Xiao, Jingjie
Si, Junqiang
Li, Xinzhi
Ma, Ketao
Zhang, Wei
Li, Li
Zhang, Yingying
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Adiponectin (APN) is an adipokine secreted from adipose tissue and exhibits biological functions such as microcirculation-regulating, hearing-protective, and antiapoptotic.
However, the effect of APN on the apoptosis of spiral arterial smooth muscle cells (SMCs) under hypoxic conditions in vitro is not clear.
We used cobalt chloride (CoCl2) to simulate chemical hypoxia in vitro, and the SMCs were pretreated with APN and then stimulated with CoCl2.
The viability of cells and apoptosis were assessed by CCK-8 and flow cytometry, respectively.
Superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, cAMP level, and the activity of PKA were detected by ELISA.
Protein expression and localization were studied by Western blot and immunofluorescence analysis.
In the present study, we found that APN exhibits antiapoptosis effects.
CoCl2 exhibited decreased cell viability, increased apoptosis and MDA levels, and decreased SOD activity in a concentration-dependent manner, compared with the control group.
Moreover, CoCl2 upregulated the expression levels of Bax and cleaved caspase-3 and then downregulated Bcl-2 levels in a time-dependent manner.
Compared with the CoCl2 group, the group pretreated with APN had increased cell viability, SOD activity, PKA activity, cAMP level, and PKA expression, but decreased MDA levels and apoptosis.
Lastly, the protective effect of APN was blocked by cAMP inhibitor SQ22536 and PKA inhibitor H 89.
These results showed that APN protected SMCs against CoCl2-induced hypoxic injury via the cAMP/PKA signaling pathway.
American Psychological Association (APA)
Xiao, Jingjie& Zhang, Yingying& Zhang, Wei& Zhang, Liang& Li, Li& Si, Junqiang…[et al.]. 2020. Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMPPKA Pathway. BioMed Research International،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136609
Modern Language Association (MLA)
Xiao, Jingjie…[et al.]. Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMPPKA Pathway. BioMed Research International No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1136609
American Medical Association (AMA)
Xiao, Jingjie& Zhang, Yingying& Zhang, Wei& Zhang, Liang& Li, Li& Si, Junqiang…[et al.]. Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMPPKA Pathway. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1136609
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136609