CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells
Joint Authors
Long, Yang
Li, Yanhui
Xu, Youhua
Zhou, Luping
Liang, Yaling
Xu, Yong
Huang, Wei
Gao, Chenlin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nuclear factor-kappa B (NF-κB) is the key part of multiple signal transduction of inflammation in the pathogenesis of diabetic nephropathy (DN).
The ubiquitin-proteasome system is extensively involved in the regulation of the NF-κB pathway.
Cylindromatosis (CYLD) has deubiquitinase activity and acts as a negative regulator of the NF-κB signaling pathway.
However, the association between CYLD and NF-κB inflammatory signaling in DN is unclear.
In the present study, mouse glomerular mesangial cells (GMCs) and rat GMCs were stimulated by elevated concentrations of glucose (10, 20, and 30 mmol/L high glucose) or mannitol as the osmotic pressure control.
CYLD was overexpressed or suppressed by transfection with a CYLD expressing vector or CYLD-specific siRNA, respectively.
Our data showed that high glucose significantly inhibited the protein and mRNA expression of CYLD in a dose- and time-dependent manner (both p<0.05).
siRNA-mediated knockdown CYLD facilitated the high glucose-induced activation of NF-κB signaling and triggered the release of MCP-1, IL-6, and IL-8 (all p<0.05).
However, these high glucose-mediated effects were blunted by overexpression of CYLD (p<0.05).
The present results support the involvement of CYLD in the regulation of NF-κB inflammatory signaling induced by elevated glucose, implicating CYLD as a potential therapeutic target of DN.
American Psychological Association (APA)
Li, Yanhui& Huang, Wei& Xu, Youhua& Zhou, Luping& Liang, Yaling& Gao, Chenlin…[et al.]. 2017. CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136632
Modern Language Association (MLA)
Li, Yanhui…[et al.]. CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1136632
American Medical Association (AMA)
Li, Yanhui& Huang, Wei& Xu, Youhua& Zhou, Luping& Liang, Yaling& Gao, Chenlin…[et al.]. CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1136632
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136632