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Overexpression of blaOXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam
Joint Authors
Nguyen, Anh T.
Pham, Son C.
Ly, Anh K.
Nguyen, Chau V. V.
Vu, Thanh T.
Ha, Tuan M.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this study was to investigate genetic structures and expression of blaOXA-58 gene in five Acinetobacter baumannii clinical isolates recovered from two hospitals in southern Vietnam during 2012-2014.
A.
baumannii isolates were identified by automated microbiology systems and confirmed by PCR.
All isolates were characterized as multidrug resistant by antimicrobial testing using the disk diffusion method.
Four imipenem susceptible and one nonsusceptible isolates (MIC>32 μg·ml-1) were identified by E-test.
PCR amplification of blaOXA-58 gene upstream and downstream sequences revealed the presence of ISAba3 at both locations in one multidrug-resistant isolate.
Semiquantitation of blaOXA-51 and blaOXA-58 gene expression was performed by the 2-ΔΔCt method.
The blaOXA-51 gene expression of five isolates showed little difference, but the isolate bearing ISAba3-blaOXA-58-ISAba3 exhibited significantly higher blaOXA-58 mRNA level.
Higher β-lactamases activity in periplasmic than cytoplasmic fraction was found in most isolates.
The isolate overexpressing blaOXA-58 gene possessed very high periplasmic enzyme activity.
In conclusion, the A.
baumannii isolate bearing ISAba3-blaOXA-58 gene exhibited high resistance to imipenem, corresponding to an overexpression of blaOXA-58 gene and very high periplasmic β-lactamase activity.
American Psychological Association (APA)
Nguyen, Anh T.& Pham, Son C.& Ly, Anh K.& Nguyen, Chau V. V.& Vu, Thanh T.& Ha, Tuan M.. 2020. Overexpression of blaOXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1136648
Modern Language Association (MLA)
Nguyen, Anh T.…[et al.]. Overexpression of blaOXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1136648
American Medical Association (AMA)
Nguyen, Anh T.& Pham, Son C.& Ly, Anh K.& Nguyen, Chau V. V.& Vu, Thanh T.& Ha, Tuan M.. Overexpression of blaOXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1136648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136648