Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco
Joint Authors
Yin, Hengfu
Wang, Jiangying
Wu, Bin
Fan, Zhengqi
Li, Xinlei
Ni, Sui
He, Libo
Li, Jiyuan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS).
Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance.
However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress.
In this study, we identified and characterized an APX (CaAPX) gene from Camellia azalea.
Quantitative real-time PCR (qRT-PCR) analysis showed that CaAPX was expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses.
Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated with CaAPX expression levels.
Furthermore, we monitored the activities of several ROS-scavenging enzymes including Cu/Zn-SOD, CAT, DHAR, and MDHAR, and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging.
Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role of CaAPX to orchestrate ROS signaling in response to temperature stresses.
Overall, this study presents a comprehensive characterization of cellular response related to CaAPX expression and provides insights to breed crops with high temperature tolerances.
American Psychological Association (APA)
Wang, Jiangying& Wu, Bin& Yin, Hengfu& Fan, Zhengqi& Li, Xinlei& Ni, Sui…[et al.]. 2017. Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco. BioMed Research International،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1136662
Modern Language Association (MLA)
Wang, Jiangying…[et al.]. Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco. BioMed Research International No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1136662
American Medical Association (AMA)
Wang, Jiangying& Wu, Bin& Yin, Hengfu& Fan, Zhengqi& Li, Xinlei& Ni, Sui…[et al.]. Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1136662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136662