Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis
Joint Authors
Pappa, Kalliopi I.
Kontostathi, Georgia
Zoidakis, Jerome
Makridakis, Manousos
Lygirou, Vasiliki
Mermelekas, George
Papadopoulos, Theofilos
Vougas, Konstantinos
Vlamis-Gardikas, Alexios
Drakakis, Peter
Vlahou, Antonia
Loutradis, Dimitrios
Anagnou, Nicholas P.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Cancer cells acquire unique secretome compositions that contribute to tumor development and metastasis.
The aim of our study was to elucidate the biological processes involved in cervical cancer, by performing a proteomic analysis of the secretome from the following informative cervical cell lines: SiHa (HPV16+), HeLa (HPV18+), C33A (HPV−), and HCK1T (normal).
Proteins were analyzed by 2D gel electrophoresis coupled to MALDI-TOF-MS.
Enrichment of secreted proteins with characteristic profiles for each cell line was followed by the identification of differentially expressed proteins.
Particularly, transforming growth factor-beta-induced protein ig-h3 (Beta ig-h3) and peroxiredoxin-2 (PRDX2) overexpression in the secretome of cancer cell lines was detected and confirmed by Western blot.
Bioinformatics analysis identified the transcription factor NRF2 as a regulator of differentially expressed proteins in the cervical cancer secretome.
NRF2 levels were measured by both Western blot and Multiple Reaction Monitoring (MRM) in the total cell extract of the four cell lines.
NRF2 was upregulated in SiHa and C33A compared to HCK1T.
In conclusion, the secreted proteins identified in cervical cancer cell lines indicate that aberrant NRF2-mediated oxidative stress response (OSR) is a prominent feature of cervical carcinogenesis.
American Psychological Association (APA)
Kontostathi, Georgia& Zoidakis, Jerome& Makridakis, Manousos& Lygirou, Vasiliki& Mermelekas, George& Papadopoulos, Theofilos…[et al.]. 2017. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis. BioMed Research International،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1136831
Modern Language Association (MLA)
Kontostathi, Georgia…[et al.]. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis. BioMed Research International No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1136831
American Medical Association (AMA)
Kontostathi, Georgia& Zoidakis, Jerome& Makridakis, Manousos& Lygirou, Vasiliki& Mermelekas, George& Papadopoulos, Theofilos…[et al.]. Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1136831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136831