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Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays
Joint Authors
Ferreira, Rafael Lopes
Zuccolotto, Tatiana
de Borba Dallagassa, Cibelle
Wielewski, Leonardo Pellizzari
Chalcoski, Barbara Maria Santano
da Silva, Mario Antonio Navarro
Richardi, Vinicius Sobrinho
Golart, Jonas
de Melo Rodovalho, Cynara
Fontana, José Domingos
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Any bioassay to test new chemically synthesized larvicides or phytolarvicides against Culicidae and more harmful mosquito species, such as Aedes aegypti and Aedes albopictus, which specifically transmit dengue, yellow fever, chikungunya viral fevers as well as Zika virus, or Anopheles gambiae, a vector for malaria and philariasis, requires thousands of well-developed larvae, preferably at the fourth instar stage.
The natural morphogenetic cycle of Aedes spp., in the field or in the laboratory, may extend to 19 days at room temperature (e.g., 25°C) from the first permanent contact between viable eggs and water and the last stage of larval growth or metamorphosis into flying adults.
Thus, accelerated sequential molting is desirable for swifter bioassays of larvicides.
We achieved this goal in Aedes aegypti with very limited strategic and low-cost additions to food, such as coconut water, milk or its casein, yeast extract, and to a lesser extent, glycerol.
The naturally rich coconut water was excellent for quickly attaining the population of instar IV larvae, the most advanced one before pupation, saving about a week, for subsequent larvicidal bioassays.
Diluted milk, as another food source, allowed an even faster final ecdysis and adults are useful for mosquito taxonomical purpose.
American Psychological Association (APA)
Fontana, José Domingos& Ferreira, Rafael Lopes& Zuccolotto, Tatiana& de Borba Dallagassa, Cibelle& Wielewski, Leonardo Pellizzari& Chalcoski, Barbara Maria Santano…[et al.]. 2020. Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays. BioMed Research International،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1136845
Modern Language Association (MLA)
Fontana, José Domingos…[et al.]. Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays. BioMed Research International No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1136845
American Medical Association (AMA)
Fontana, José Domingos& Ferreira, Rafael Lopes& Zuccolotto, Tatiana& de Borba Dallagassa, Cibelle& Wielewski, Leonardo Pellizzari& Chalcoski, Barbara Maria Santano…[et al.]. Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1136845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136845