Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPKNF-κB Pathways in Rats

Abstract EN

Schisandrin B (ScB), isolated from Schisandra chinensis (S.

chinensis), is a traditional Chinese medicine with proven cardioprotective and neuroprotective effects.

However, it is unclear whether ScB also has beneficial effects on rat hind limb ischemia/reperfusion (I/R) injury model.

In this study, ScB (20 mg/kg, 40 mg/kg, and 80 mg/kg) was administered via oral gavage once daily for 5 days before the surgery.

After 6 h ischemia and 24 h reperfusion of left hind limb, ScB reduced I/R induced histological changes and edema.

ScB also suppressed the oxidative stress through decreasing MDA level and increasing SOD activity.

Moreover, above changes were associated with downregulated TNF-α mRNA expression and reduced level of IL-1β in plasma.

Meanwhile, ScB treatment downregulated activation of p38MAPK, ERK1/2, and NF-κB in ischemic skeletal muscle.

These results demonstrate that ScB treatment could prevent hind limb I/R skeletal muscle injury possibly by attenuating oxidative stress and inflammation via p38MAPK, ERK1/2, and NF-κB pathways.