Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma
Joint Authors
Song, Yuxuan
Jin, Donghui
Chen, Yuan
Zhang, Peng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Lung cancer is the most common cancer and the most common cause of cancer-related death worldwide.
However, the molecular mechanism of its development is unclear.
It is imperative to identify more novel biomarkers.
Methods.
Two datasets (GSE70880 and GSE113852) were downloaded from the Gene Expression Omnibus (GEO) database and used to identify the differentially expressed genes (DEGs) between lung cancer tissues and normal tissues.
Then, we constructed a competing endogenous RNA (ceRNA) network and a protein-protein interaction (PPI) network and performed gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and survival analyses to identify potential biomarkers that are related to the diagnosis and prognosis of lung cancer.
Results.
A total of 41 lncRNAs and 805 mRNAs were differentially expressed in lung cancer.
The ceRNA network contained four lncRNAs (CLDN10-AS1, SFTA1P, SRGAP3-AS2, and ADAMTS9-AS2), 21 miRNAs, and 48 mRNAs.
Functional analyses revealed that the genes in the ceRNA network were mainly enriched in cell migration, transmembrane receptor, and protein kinase activity.
mRNAs DLGAP5, E2F7, MCM7, RACGAP1, and RRM2 had the highest connectivity in the PPI network.
Immunohistochemistry (IHC) demonstrated that mRNAs DLGAP5, MCM7, RACGAP1, and RRM2 were upregulated in lung adenocarcinoma (LUAD).
Survival analyses showed that lncRNAs CLDN10-AS1, SFTA1P, and ADAMTS9-AS2 were associated with the prognosis of LUAD.
Conclusion.
lncRNAs CLDN10-AS1, SFTA1P, and ADAMTS9-AS2 might be the biomarkers of LUAD.
For the first time, we confirmed the important role of lncRNA CLDN10-AS1 in LUAD.
American Psychological Association (APA)
Jin, Donghui& Song, Yuxuan& Chen, Yuan& Zhang, Peng. 2020. Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma. BioMed Research International،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1136973
Modern Language Association (MLA)
Jin, Donghui…[et al.]. Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma. BioMed Research International No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1136973
American Medical Association (AMA)
Jin, Donghui& Song, Yuxuan& Chen, Yuan& Zhang, Peng. Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1136973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1136973